Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

Journal: Archives of Medical Science : AMS

Article Title: Therapeutic potential of mesenchymal stem cells in cisplatin-induced acute kidney injury via ASK-1/TXNIP pathway modulation

doi: 10.5114/aoms/193707

Figure Lengend Snippet: Immunohistochemical staining of VEGF of renal cortex sections at magnification ×400, bar 25 µm. A – Immunohistochemical-stained kidney sections of control group showing strong reaction of VEGF protein expression in the renal cortex. B – Immunohistochemical-stained kidney sections of AKI group showing significant reduction in the expression of VEGF in renal medulla. C – Immunohistochemical-stained kidney sections for VEGF protein expression of FUR group showing mild increase in the expression of VEGF in renal cortex. D – Immunohistochemical-stained kidney sections for VEGF protein expression of MSCs group showing marked increase in the expression of VEGF in renal medulla E – The angiogenic effects of MSC in kidney tissue, expressed as area %. Data were presented as means ± SEM. Each column represented the means ± SEM. The mean variations between the groups using Turkey’s significant difference test. a p < 0.01 vs. control, b p < 0.01 vs. cisplatin induced acute kidney injury cells, c p < 0.01 vs. furosemide treated cells

Article Snippet: Hematoxylin and eosin (H&E) stain was applied to 5 μm sections of paraffin-embedded renal tissues for histological investigation [ ], using anti-VEGF rabbit polyclonal Ab (Boster Biological Technology, Pleasanton, CA, USA, Cat.# PA1080).

Techniques: Immunohistochemical staining, Staining, Control, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Co-delivery of endometrial mesenchymal stem cells and macrophages by an electrospun patch promotes angiogenesis during endometrial injury repair via VEGF related signalling

doi: 10.1186/s13287-026-04929-2

Figure Lengend Snippet: Assessment of the tube formation capability, CD31 and VEGF gene expressions for HUVECs treated without (control) and with cell culture supernatant collected from H-EMSCs seeded on PCL-HA (PCL-HA/H-E) or H-EMSCs and mø co-seeded on PCL-HA (PCL-HA/H-E/mø). A Tube formation assay of HUVECs in the control, PCL-HA/H-E and PCL-HA/H-E/mø groups. Scale bar = 200 μm. B Quantification of the total length of the tubes formed. C Quantification of the total number of meshes formed. D Quantification of the number of nodes formed. E CD31 gene expression of the HUVECs. F VEGF gene expression of the HUVECs. *** p < 0.001. ## p < 0.01, ### p < 0.001 vs. Control

Article Snippet: The following lists the primary and secondary antibodies used: rabbit polyclonal to CD31 (1:200, 28083-1-AP, Proteintech); rabbit polyclonal to VEGF (1:200, PB9071, Boster); AlexaFluor ® 488 goat anti-mouse IgG (1:200, A28175, Thermo scientific) and AlexaFluor ® 568 goat anti-rabbit IgG (1:200, A-11011, Thermo scientific).

Techniques: Control, Cell Culture, Tube Formation Assay, Gene Expression

Journal: Stem Cell Research & Therapy

Article Title: Co-delivery of endometrial mesenchymal stem cells and macrophages by an electrospun patch promotes angiogenesis during endometrial injury repair via VEGF related signalling

doi: 10.1186/s13287-026-04929-2

Figure Lengend Snippet: Assessment of the tube formation capability, CD31 and VEGF gene expressions of HUVECs treated with cell culture supernatant collected from H-EMSCs seeded on PCL-HA (PCL-HA/H-E) or H-EMSCs and mø co-seeded on PCL-HA (PCL-HA/H-E/mø) or H-EMSCs and mø co-seeded on PCL-HA and VEGF inhibitor (PCL-HA/H-E/mø/VEGF inhibitor). A Tube formation assay of HUVECs in the PCL-HA/H-E, PCL-HA/H-E/mø and PCL-HA/H-E/mø/VEGF inhibitor groups. Scale bar = 200 μm. B Quantification of the total length of the tubes formed. C Quantification of the total number of meshes formed. D Quantification of the number of nodes formed. E CD31 gene expression of the HUVECs. F VEGF gene expression of the HUVECs. *** p < 0.001. # p < 0.05, ### p < 0.001 vs. PCL-HA/H-E group

Article Snippet: The following lists the primary and secondary antibodies used: rabbit polyclonal to CD31 (1:200, 28083-1-AP, Proteintech); rabbit polyclonal to VEGF (1:200, PB9071, Boster); AlexaFluor ® 488 goat anti-mouse IgG (1:200, A28175, Thermo scientific) and AlexaFluor ® 568 goat anti-rabbit IgG (1:200, A-11011, Thermo scientific).

Techniques: Cell Culture, Tube Formation Assay, Gene Expression

Journal: Stem Cell Research & Therapy

Article Title: Co-delivery of endometrial mesenchymal stem cells and macrophages by an electrospun patch promotes angiogenesis during endometrial injury repair via VEGF related signalling

doi: 10.1186/s13287-026-04929-2

Figure Lengend Snippet: VEGF, CD31 gene and protein expression of rat endometrial tissues of the PCL-HA/H-E, PCL-HA/H-E/mø and PCL-HA/H-E/mø/VEGF inhibitor groups at day 7. A VEGF gene expression. B Quantification of VEGF protein concentration (pg/ml). C Representative images of VEGF immunostaining. Cell nuclei stained in blue while VEGF stained in red. Scale bar = 100 μm. D Quantification of VEGF staining area (%). E CD31 gene expression. F Representative images of CD31 immunostaining. Cell nuclei stained in blue while CD31 stained in red. Scale bar = 100 μm. G Quantification of CD31 staining area (%). *** p < 0.001. ## p < 0.01, ### p < 0.001 vs. PCL-HA/H-E group

Article Snippet: The following lists the primary and secondary antibodies used: rabbit polyclonal to CD31 (1:200, 28083-1-AP, Proteintech); rabbit polyclonal to VEGF (1:200, PB9071, Boster); AlexaFluor ® 488 goat anti-mouse IgG (1:200, A28175, Thermo scientific) and AlexaFluor ® 568 goat anti-rabbit IgG (1:200, A-11011, Thermo scientific).

Techniques: Expressing, Gene Expression, Protein Concentration, Immunostaining, Staining